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產品分類 > 真菌過敏原 > Alt a 1 ELISA kit (EL-AA1)鏈格孢過敏原1檢測試劑盒

Alt a 1 ELISA kit (EL-AA1)鏈格孢過敏原1檢測試劑盒

產品貨號:EL-AA1
促銷: 12720.00
價格: 12720.00

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kit
美國Indoor
ELISA kit for measuring Alternaria alternata allergen, Alt a 1.

Antibodies:2C10/3B6

Sensitivity:0.2ng/ml

Limit of Detection:0.4ng/ml

Assay Performance:Inquire

Contents:

Monoclonal antibody 2C10, rAlt a 1 Standard, Biotinylated antibody 3B6; sufficient reagents for 10 microtiter plates.

Storage:Store at 4°C.

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:

EL-AA1 Certificate of Analysis

Focus on... Alt a 1

Letter on new antibodies in Alt a 1 ELISA kit

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:2C10 (clone 2C10 G4)

Vial:Red Cap; 100μl

Immunogen:Alt a 1

Isotype:Mouse IgG

Specificity:Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.

Purification:Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.

Concentration:1mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



Certificate of Analysis

Monoclonal Antibody:3B6 (clone 3B6 H9)

Vial:Brown Cap; 100μl

Immunogen:rAlt a 1

Isotype:Mouse IgG

Specificity:Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.

Purification:Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



Certificate of Analysis

Allergen Standard:rAlt a 1

Vial:White Cap; 400μl

Composition:Recombinant Alt a 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.

Concentration:1000ng/ml Alt a 1

Calibration:The rAlt a 1 was purified from Pichia pastoris by size exclusion HPLC. The protein content was determined by amino acid analysis.

References:
1. Vailes L, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman M. Quantification of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J Allergy Clin Immunol 2001;107(4):641-6. 
 
2. Unger A, Stoger P, Simon-Nobbe B, Susani M, Crameri R, Ebner C et al. Clinical testing of recombinant allergens of the mold Alternaria alternata. Int Arch Allergy Immunol 1999;118(2-4):220-1. 
 
3. Asturias JA, Ibarrola I, Ferrer A, Andreu C, Lopez-Pascual E, Quiralte J et al. Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens. J Allergy Clin Immunol 2005;115(6):1210-7. 
 
4. Chruszcz M, Chapman MD, Osinski T, Solberg R, Demas M, Porebski PJ et al. Alternaria alternata allergen Alt a 1: A unique beta-barrel protein dimer found exclusively in fungi. J Allergy Clin Immunol 2012;130(1):241-7.
(For complete reference list see EL-AA1 Certificate of Analysis)


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