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產品分類 > 屋塵螨過敏原 > Der p 1 ELISA kit (EL-DP1A)屋塵螨過敏原1檢測試劑盒

Der p 1 ELISA kit (EL-DP1A)屋塵螨過敏原1檢測試劑盒

產品貨號:EL-DP1A
促銷: 12720.00
價格: 12720.00

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kit
美國Indoor
ELISA kit for measuring D. pteronyssinus allergen, Der p 1.

Antibodies:10B9/5H8

Sensitivity:0.5ng/ml

Limit of Detection:2ng/ml

Assay Performance:Inquire

Contents:

Monoclonal antibody 10B9, Universal Allergen Standard, Biotinylated antibody 5H8;

sufficient reagents for 10 plates.

Storage:Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6

Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine Serum Albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:EL-DP1A Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:10B9 (clone 10B9 F6 A12)

Vial:Red Cap; 100μl

Immunogen:Der p 1

Isotype:Mouse IgG1

Specificity:Binds to an epitope on dust mite Dermatophagoides pteronyssinus allergen, Der p 1.

Purification:Produced in ascites and purified by ammonium sulphate precipitation and affinity

chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

Concentration:1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml),

0.22μm filtered, preservative free.


Certificate of Analysis

Monoclonal Antibody:5H8 (clone 5H8 C12 D8)

Vial:Brown Cap; 100μl

Immunogen:Der p 1

Isotype:Mouse IgG2A

Specificity:Binds to an epitope on dust mite Dermatophagoides pteronyssinus allergen, Der p 1.

Purification:Produced in cell culture and purified by affinity chromatography using Protein A.

Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1%

BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



Certificate of Analysis

Allergen Standard:Universal Allergen Standard

Vial:White Cap; 400μl

Composition:A formulation of eight purified natural allergens prepared in 1% BSA/50%

glycerol/PBS, pH 7.4

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)


Der p 1

Amino-acid analysis2500
Der f 1

Amino-acid analysis

2500


Der p 2

Amino-acid analysis

1000


Fel d 1

Amino-acid analysis

1000


Can f 1

Amino-acid analysis

2500


Rat n 1

Amino-acid analysis

1000


Mus m 1

Amino-acid analysis

250


Bla g 2

Amino-acid analysis

2500


References:

1.  Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods 1989; 118(2):227-235.

2.  Custovic A, Taggart SC, Francis HC, Chapman MD, Woodcock A. Exposure to house dust mite allergens and the clinical activity of asthma. J Allergy Clin Immunol 1996; 98(1):64-72.

3.  Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

4.  Sawyer G, Kemp T, Shaw R et al. Biologic pollution in infant bedding in New Zealand: high allergen exposure during a vulnerable period. J Allergy Clin Immunol 1998; 102(5):765-770.

5.  Arbes SJ, Jr., Cohn RD, Yin M et al. House dust mite allergen in US beds: results from the First National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414.

6.  Gruchalla RS, Pongracic J, Plaut M et al. Inner City Asthma Study: relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005; 115(3):478-485.

7.  Zock JP, Heinrich J, Jarvis D et al. Distribution and determinants of house dust mite allergens in Europe: the European Community Respiratory Health Survey II. J Allergy Clin Immunol 2006; 118(3):682-690.

8.  Jarvis D, Zock JP, Heinrich J et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol 2007; 119:697-704.

9.  Earle CD, King EM, Tsay A et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 2007; 119(2):428-433.

(For complete reference list see EL-DP1 Certificate of Analysis)


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