Product Number: 30000
Melanoma differentiation-associated protein 5 (MDA5; also known as clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays.
Binds IgG-type human auto-antibodies.
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Full-length cDNA coding for the human melanoma differentiation-associated protein 5 fused to a deca-histidine purification tag.
SDS-PAGE (purity > 80%); Western blot with i: anti-MDA5 positive sera;
ii: monoclonal anti-His-tag antibody.
Calculated molecular weight:
Calculated isoelectric point:
Immunological tests/Functionality: Immunodot test with positive/negative sera panels.
Recommended buffer/storage and handling conditions:
Recommendations for storage buffer: ionic strength between 400 and 600 mM salt, neutral to slightly alkaline pH and 6M Urea.
–70° to –80° C. Repeated freeze/thaw cycles should be avoided.