Product Number: 11100
Human Glycyl-tRNA Synthetase (EC 188.8.131.52; EJ). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays.
Binds IgG-type human auto-antibodies.
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Full-length cDNA coding for the human Glycyl-tRNA Synthetase fused to a deca-histidine purification tag.
SDS-PAGE (purity > 80%); Western blot with i: anti-EJ positive sera; ii: monoclonal anti-His-tag antibody.
Calculated molecular weight:
Calculated isoelectric point:
Immunological tests/Functionality: Immunodot test with positive/negative sera.
Recommended buffer/storage and handling conditions:
Recommendations for storage buffer: ionic strength between 100 and 200 mM salt, neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent. Storage temperature:
–70° to –80° C. Repeated freeze/thaw cycles should be avoided.
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